Journal: Journal of innate immunity

Article Title: Cross-talk between human dendritic cell subsets influences expression of RNA sensors and inhibits picornavirus infection.

doi: 10.1159/000300568

Figure Lengend Snippet: Fig. 2. Human DC subsets express different profiles of viral nu- cleic acid receptors. a Mean expression (+SD) of TLR3, TLR7 and TLR8 in human moDCs, freshly isolated mDCs and freshly iso- lated pDCs. RNA was isolated from unstimulated cells and TLR mRNA transcript levels determined using conventional qPCR. Expression levels relative to PBGD. * p ! 0.05; * * p ! 0.01. b Ex- pression levels of RIG-I, MDA5, PKR and LGP2 in resting human DC subsets were determined as described for a . Shown is mean expression + SD. * p ! 0.05; * * p ! 0.01. c Confocal laser scanning microscopy analysis of MDA5 expression in human moDCs and pDCs. Unstimulated cells were stained using antibodies against

Article Snippet: Anti-RIG-I and anti-PKR antibodies were purchased from ProSci Incorporated and Becton Dickinson Transduction Laboratories, respectively.

Techniques: Expressing, Isolation, Confocal Laser Scanning Microscopy, Staining

Journal: Translational Psychiatry

Article Title: Carboxypeptidase E conditional knockout mice exhibit learning and memory deficits and neurodegeneration

doi: 10.1038/s41398-023-02429-y

Figure Lengend Snippet: a – e Western blot analysis of p -TrkB, BDNF, p -mTOR, mTOR, p -AKT, and AKT levels in the hippocampus. f Immunofluorescence of CPE, MAP2, DCX, and GFAP; and the relative fluorescent intensities of g CPE in Sub, h MAP2 in Sub, i MAP2 in hilius, j DCX in DG, k GFAP in DG of WT, CPE flox/− , and CPE flox/flox mice at 100× and 400× (square in the panel). n = 6; * P < 0.05 and ** P < 0.01 compared with WT; values are mean ± SEM.

Article Snippet: After blocking with 5% nonfat milk, the membrane was blotted with antibodies against mouse anti-β-actin antibody (1:1000, CST, 4967S), mouse anti-CPE antibody (1:1000; BD bioscience, 610758), mouse anti-BDNF antibody (1:600, Abcam, UK, ab108319), rabbit anti-p-TrkB antibody (1:1000; Boster, Wuhan, China, BM4437), rabbit anti-AKT (1:2000, Cell Signaling Technology, 4691S), and anti-p-AKT (1:2000, Cell Signaling Technology, 23430S) overnight at 4 °C.

Techniques: Western Blot, Immunofluorescence